Process analytical technology

Online process analysis of liquids

​​​Keit provides real-time process analysis of liquids with the IRmadillo process analyzer. The process FTIR spectrometer is built vibration-proof with an in situ probe enabling the instrument to be mounted directly onto, or in-line with, reaction vessels for real-time results.

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The IRmadillo is inherently compact, low maintenance, immune to vibration, and certified safe for use in hazardous environments. It is an effective analytical instrument for monitoring and control of liquid-state reactions at the point of production including the analyses of oil, acids, sugars, alcohols, proteins, contaminants, chemicals, biofuels and petroleum products.

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Advantages:

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• Confidence to know when your reactions have peaked - not after they have peaked
• Complete understading of your reactions through simultaneous, multi-component analysis
• Ease of use with tough, long-term stability
• Faster production decisions through online, real-time analysis

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See more about applications here​

Gasanalyzers

​BioPAT® Xgas 

​​The BioPAT® Xgas from Sartorius Bioprocess Solutions monitors and controls bioprocesses in real time. It is a combined oxygen-carbon dioxide sensor featuring automatic compensation of humidity and pressure. The range for oxygen is 1 - 50% and for carbon dioxide 0 - 10%, with ±3% accuracy on the reference value and a difference of less than ±0.2% on the reading. The sensor is non-invasive and can be conveniently integrated onto a new Sartorius Stedim bioreactor exhaust system.

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The advantages of using BioPAT® Xgas are the following:

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• Parallel and continuous measurement of O2 and CO2 using just one sensor
• Standardized integration into Sartorius bioreactors for event-based control
• High accuracy and process reliability ensured by automatic compensation of humidity and pressure
• Compact design for convenient integration into both small lab bioreactors and process-scale fermenters
• Status display and calibration buttons directly on the BioPAT® Xgas for easy operation

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See more about ​BioPAT® Xgas here

Online monitoring of glucose

​BioPAT® Trace is used in laboratories for simultaneous online monitoring of glucose and lactate, e.g. for industrial cultivation of microorganisms or mammalian cells. 

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The BioPAT® Trace provides continuous online process analysis regardless of the cultivation type (batch/fed-batch). The BioPAT® Trace can provide online analysis of up to 4 bioreactors simultaneously.

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The BioPAT ® Trace has three sampling options available: filtration probe, dialysis probe and bypass block. These options all enable sterile removal from the bioreactor.

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Online monitoring of glucose/lactate means:

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• 24/7 monitoring of the process
• Short response time
• Control based on measurements ensures a stable glucose level (50mg/l - 2g/l) in the medium
• Possible to check conditions for optimum cultivation

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There are two different sensors. A filter probe, where the sample is taken out and analyzed in the apparatus, and a dialysis probe for direct measurement of the glucose/lactate concentration. The probes are available for 12 mm, 19 mm and 25 mm ports. Both types are of course suitable for sterile sampling.

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The analysis functions in the BioPAT® Trace are based on a combination of enzymatic reactions and anode oxidation on which glucose and lactate concentrations can be detected as function of the conductivity.

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Datasheet BioPAT® Trace and BioPAT® Multi Trace

Turbidity measurement

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BioPAT® Fundalux

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The BioPAT® Fundalux is an optical piece of equipment for online turbidity measurement. The probe operates on a transmittance principle using IR light to track total biomass. 

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It is a tool to increase microbial fermentation yield by maximizing the use of nutrients, gases and other critical parameters. The system reduces the cost associated with 24-hour sampling and removes inconsistent offline analysis. The BioPAT® Fundalux comes integrated into Sartorius' BioPAT® DCU and automatic data conversion to optical density can be achieved with BioPAT® MFCS.

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See the range of probes here

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​BioPAT® ViaMass

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BioPAT® ViaMass is a system from Sartorius Bioprocess Solutions for measuring viable biomass in cell culture, yeast and microbial processes. Unlike known reference methods, this system is completely integrated into single-use bioreactors. It determines the biomass directly, thus reducing the need for manual sampling. Integrated into the bioreactor’s DCU control system, BioPAT® ViaMass permits automatic monitoring and control of viable biomass.

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Using BioPAT® ViaMass together with the BioPAT® MFCS family delivers decisive advantages, particularly for developing control strategies.

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Thanks to real-time information on viable biomass during fermentation, time-consuming and contamination-prone sampling is now a thing of the past.

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The following advantages are obtained:

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• Reduces the need for manual sampling
  
• Real-time measurement of and feedback on viable biomass
• Ensures batch-to-batch consistency
• Eliminates operator influence that occurs in manual biomass measurements