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Bioreactors and fermenters

Søren Bidstrup

Product specialist and Managing Director

E-mail: sb@aagechristensen.dk

Mobile: +45​ 40 45 60 10

Michael Siewartz Nielsen

Product specialist

E-mail: msn@aagechristensen.dk

Mobile: +45​ 40 37 21 38

At Aage Christensen you find a wide range of bioreactors and fermenters from ​Sartorius Bioprocess Solutions for lab, pilot- and process-scale applications. The Biostat® and ambr® product series consist of bioreactors for single use as well as autoclavable and in-situ sterilizable systems.

We supply fermenters for all types of applications, so please do not hesitate to contact us​ if you wish to learn more or if you wish to buy. Call us on +45 36 44 24 44 so that we can discuss your needs.

Below you will find an overview of the range of fermenters we offer.

Microbioreactors and fermenters from TAP Biosystems

TAP Biosystems, a leading global provider of automated cell culture systems and fermenters for life science research, development and production, is now part of Sartorius Stedim Biotech.

​ambr® 15 cell culture

​ambr® 15 cell culture is an automated microscale bioreactor system that replicates classic laboratory-scale bioreactors.

It is an automated workstation offering parallel control of 24 or 48 bioreactor experiments each with a working volume of up to 15 ml. It is designed for easy installation in a standard laminar flow hood allowing aseptic operation. With ambr® 15 it is possible to make effective and scalable experiments compared to the large volumes which are normally necessary in classic bioreactors, and the system has the following advantages:

  • pH, pO2 mixing and temperature can be controlled
  • High efficiency as 24 or 48 reactors can be controlled parallelly
  • Large number of experiments with minimum space requirements
  • Small volumes causing reduced media consumption
  • Helps to create better cell lines faster by automatization of set-up, feeding, bottom addition and sampling

Learn more about​ ambr® 15 cell culture here

​ambr® 15 fermentation

​ambr® 15 fermentation is an automated microscale bioreactor system designed for microbial fermentation. Based on the gold-standard ambr 15 technology, it provides a microscale system for high throughput microbial screening with fed-batch culture capability.

With an ambr® 15 fermentation system productivity is increased by automating experiments, sampling and reagent addition for up to 24 experiments in parallel each with a working volume of 10-15 ml.

Compared to shaking flasks you here obtain full control of stirring, DO and pH as well as possibility of working with fed-batch.

Learn more about ​ambr® 15 fermentation here

ambr® 250 modular

ambr® 250 modular is a high-performance bioreactor system for parallel microbial or mammalian fermentation in 100-250 ml single-use bioreactors. ambr® 250 modular utilizes the same advanced and innovative bioreactor technology pioneered in the original ambr® 250 high throughput system. The system is modularly built. It comprizes a control module and up to 4 fermentation modules with room for 2 bioreactors per module. ambr® 250 modular can be accessed through the integrated screen on the control module and is configured with intuitive system software.

Learn more about ambr® 250 modular

ambr® 250 high throughput

ambr® 250 high throughput is an improved parallel microbial and cell culture system for 12 or 24 bioreactors. The system utilizes 100-250 ml single-use vessels and is fully automatic.

When using ambr® 250 high throughput, productivity is drastically improved, as trials can be carried out at a fraction of the costs compared with traditional bioreactors and higher flow is obtained. The system makes it possible to get a quick evaluation of a long range of conditions in a realistic bioreactor environment with full control of all cultivation conditions.

It gives a high throughput scale-down model to research a long range of conditions and strains with excellent scalability to bioreactors and fermenters. Furthermore the costs for infrastructure are redused by removing the need for cleaning and sterilization facilities.

Learn more about ambr® 250 high throughput

ambr​ analysis module for ambr 15 and ambr 250

The new analysis module can be connected to existing ambr systems and measures at-line pH and Raman spectroscopy.

  • Continuous in-process recalibration of pH
  • Reduced CO2 degassing deviation compared with off-line pH measurement
  • Integration of Raman spectroscopy from Kaiser Optical or Tornado Spectral via Sartorius' fiber optical sensor
  • Small sample volumes (60 µL) allows frequent pH measurement

Learn more about the ambr analysis module

Raman spectroscopy - NEWS

Raman spectroscopy for ambr 15 / ambr 250 is a fast and easy process. With Raman spectroscopy you get automated data integration with ambr.

Based on Raman data it is possible to automate the control of e.g. glucose in ambr bioreactor.

  • All key data are automatically collected in the ambr software
  • Small sample volumes - 160 µL for ambr 15 and 200 µL for ambr 250
  • Fast analysis time - 10-15 minutes per sample

Learn more about Raman spectroscopy here

A​utoclavable fermenters

From Sartorius Bioprocess Solutions we offer fermenters with autoclavable or single-use culture vessels with a wide range of different working volumes. They are used for cell culture or microbial fermentation as well as for batch and fed-batch processes. It is possible to control temperature, stirrer speed, pH, pO2, level and afoam. Aeration is available in 2 versions, with air/O2 ​for microbial fermentation and air/O2/N2/CO2 for mammalian fermentation. The vessels can also be adapted to individual applications (ring sparger, microsparger, rushton impellers, marine impellers etc.).


The range of autoclavable fermenters and bioreactors includes:

In-situ sterilizable bioreactor systems

From Sartorius Bioprocess Solutions we offer in-situ sterilizable bioreactor systems with working volumes from 2 L to 200 L, suitable for pilot-scale process development and for production. The systems can be used for batch and fed-batch fermentation and perfusion. The integrated pumps control acid, base, level and afoam. In addition, 2 external pumps can be added (e.g for substrate feed). 

Sterilization is fully automatic by pushing a button where both sterilization time and end temperature are defined by the user. Aeration is done with air/O2​ (microbial application) and ​air/O2/CO2/N2​ (mammalian application) via the integrated mass flow controllers. The temperature control is fully automatic using steam and cooling water.

​The range of in-situ bioreactors includes:

Single use bioreactors

From Sartorius Bioprocess Solutions we offer bags which are suitable for working volumes between 0.5 and 2,000 L. Systems allowing working volumes up to 250 L consist of a rocking unit with a bag holder, a control unit and a bag. The bags are installed on the platform which rocks, and this wave motion ensures a constant smooth mixing in the bag while the medium is aerated (air, O2, CO2 and N2). With single use electrodes it is posible to measure and control pH and pO2 during fermentation. The temperature can be adjusted with a heating jacket, or with a cooling coil (option). 

 ​

For working volumes from 12.5 -2,000 L we can offer systems with integrated stirrers in the bags and stainless steel holders for holding the bags during cultivation. 

Applications:

  • Single-use fermenters for mammalian and microbial applications
  • Expansion and differentiation of stem cells
  • Microorganisms for cultivation with low to medium density and low oxygen consumption
  • Transfer of shaking or tissue culture flasks to larger volumes
  • Inoculation reactor in larger scale - both GMP and non-GMP
  • CO2 incubator

Advantages:

  • No sterilization and cleaning
  • Minimal "downtime" between fermentations
  • The same platform can be used for different volumes
  • User friendly touchscreen reducing training to a minimum
  • Upscalable process

The range of single use cultivation bag systems includes:

Process control of our fermenters

​Data acquisition, monitoring and control of fermentation processes.

BioPAT® MFCS/win is a Windows-based SCADA software for control, data acquisition and data processing from fermentation processes used all over the world. With more than 3,000 installations and 25 years of experience the GAMP 4 category system can meet basically all specific wishes and requirements. The system is modularly built, enabling you to choose the features you need:


Recipe Control:

Allows you to program reproducible fermentation processes.


21CFR Part 11:

​Expands the MFCS/win SCADA system to meet FDA's 21CFR Part 11 guidelines.


Terminal Server: Allows connection to MFCS/win server from office or home PC (workstation). 

Remote Alarming: Transfers alarms to mobile phone or e-mail.

The most important feature of the system is of course acquisition of fermentation data for later analysis purposes. If you wish to control fermentations via a computer, this is possible with the recipe module (S88-recipe) with which the whole fermentation process can be preprogrammed.

Courses in BioPAT® MFCS/win

We offer BioPAT​® MFCS/win courses. When these are to be held are determined individually. 

To learn more about our training program click on the link below.

Basic course MFCS/win 3.1 User Training program