Bioreactors and fermenters
Quality
Sartorius delivers state-of-the-art equipment of the highest quality which means that customers get the best solution in the market and that downtime is kept at a minimum.
Local service
Aage Christensen has a local service team in Scandinavia with great expertise in maintenance of fermenters as well as a local stock of spare parts which ensures quick service in case of breakdown.
Great expertise
Aage Christensen has two experienced fermenter specialists who are ready to help you find the best solution.
Sartorius is one of the leading suppliers of upstream and downstream equipment within biotechnology and the pharmaceutical industry. The company is known for delivering state-of-the-art equipment of the highest quality. Aage Christensen is Sartorius’ partner in selling fermenters for microbial and mammalian fermentation in Scandinavia. Here you will find an overview of the range of fermenters we offer.
Microbioreactors and fermenters from TAP Biosystems
TAP Biosystems, a leading global provider of automated cell culture systems and fermenters for life science research, development and production, is now part of Sartorius Stedim Biotech.
ambr® 15 cell culture
ambr® 15 cell culture is an automated microscale bioreactor system that replicates classic laboratory-scale bioreactors.
It is an automated workstation offering parallel control of 24 or 48 bioreactor experiments each with a working volume of up to 15 ml. It is designed for easy installation in a standard laminar flow hood allowing aseptic operation. With ambr® 15 it is possible to make effective and scalable experiments compared to the large volumes which are normally necessary in classic bioreactors, and the system has the following advantages:
- pH, pO2 mixing and temperature can be controlled
- High efficiency as 24 or 48 reactors can be controlled parallelly
- Large number of experiments with minimum space requirements
- Small volumes causing reduced media consumption
- Helps to create better cell lines faster by automatization of set-up, feeding, bottom addition and sampling
ambr® 250 modular
ambr® 250 modular is a high-performance bioreactor system for parallel microbial or mammalian fermentation in 100-250 ml single-use bioreactors. ambr® 250 modular utilizes the same advanced and innovative bioreactor technology pioneered in the original ambr® 250 high throughput system. The system is modularly built. It comprizes a control module and up to 4 fermentation modules with room for 2 bioreactors per module. ambr® 250 modular can be accessed through the integrated screen on the control module and is configured with intuitive system software.
ambr® 250 high throughput
ambr® 250 high throughput is an improved parallel microbial and cell culture system for 12 or 24 bioreactors. The system utilizes 100-250 ml single-use vessels and is fully automatic.
When using ambr® 250 high throughput, productivity is drastically improved, as trials can be carried out at a fraction of the costs compared with traditional bioreactors and higher flow is obtained. The system makes it possible to get a quick evaluation of a long range of conditions in a realistic bioreactor environment with full control of all cultivation conditions.
It gives a high throughput scale-down model to research a long range of conditions and strains with excellent scalability to bioreactors and fermenters. Furthermore the costs for infrastructure are redused by removing the need for cleaning and sterilization facilities.
ambr-analysemodul til ambr 15 og ambr 250
The new analysis module can be connected to existing ambr systems and measures at-line pH and Raman spectroscopy.
- Løbende in-process pH-rekalibrering
- Reduced CO2 degassing deviation compared with off-line pH measurement
- Integration of Raman spectroscopy from Kaiser Optical or Tornado Spectral via Sartorius’ fiber optical sensor
- Small sample volumes (60 µL) allows frequent pH measurement
Raman spectroscopy
Raman spectroscopy for ambr 15 / ambr 250 is a fast and easy process. With Raman spectroscopy you get automated data integration with ambr.
Based on Raman data it is possible to automate the control of e.g. glucose in ambr bioreactor.
- All key data are automatically collected in the ambr software
- Small sample volumes – 160 µL for ambr 15 and 200 µL for ambr 250
- Fast analysis time – 10-15 minutes per sample
Autoclavable fermenters
From Sartorius Bioprocess Solutions we offer fermenters with autoclavable or single-use culture vessels with a wide range of different working volumes. They are used for cell culture or microbial fermentation as well as for batch and fed-batch processes. It is possible to control temperature, stirrer speed, pH, pO2, level and afoam. Aeration is available in 2 versions, with air/O2 for microbial fermentation and air/O2/N2/CO2 for mammalian fermentation. The vessels can also be adapted to individual applications (ring sparger, microsparger, rushton impellers, marine impellers etc.).
The range of autoclavable fermenters and bioreactors includes:
In-situ sterilizable bioreactor systems
From Sartorius Bioprocess Solutions we offer in-situ sterilizable bioreactor systems with working volumes from 2 L to 200 L, suitable for pilot-scale process development and for production. The systems can be used for batch and fed-batch fermentation and perfusion. The integrated pumps control acid, base, level and afoam. In addition, 2 external pumps can be added (e.g for substrate feed).
Sterilization is fully automatic by pushing a button where both sterilization time and end temperature are defined by the user. Aeration is done with air/O2 (microbial application) and air/O2/CO2/N2 (mammalian application) via the integrated mass flow controllers. The temperature control is fully automatic using steam and cooling water.
The range of in-situ bioreactors includes:
Single use bioreactors
From Sartorius Bioprocess Solutions we offer bags which are suitable for working volumes between 0.5 and 2,000 L. Systems allowing working volumes up to 250 L consist of a rocking unit with a bag holder, a control unit and a bag. The bags are installed on the platform which rocks, and this wave motion ensures a constant smooth mixing in the bag while the medium is aerated (air, O2, CO2 and N2). With single use electrodes it is possible to measure and control pH and pO2 during fermentation. The temperature can be adjusted with a heating jacket, or with a cooling coil (option).
For working volumes from 12.5 -2,000 L we can offer systems with integrated stirrers in the bags and stainless steel holders for holding the bags during cultivation.
Applications:
- Single-use fermenters for mammalian and microbial applications
- Expansion and differentiation of stem cells
- Microorganisms for cultivation with low to medium density and low oxygen consumption
- Transfer of shaking or tissue culture flasks to larger volumes
- Inoculation reactors in larger scale – both GMP and non-GMP
- CO2 incubators
Advantages:
- No sterilization and cleaning
- Minimal “downtime” between fermentations
- The same platform can be used for different volumes
- User friendly touchscreen reducing training to a minimum
- Upscalable process
The range of single use cultivation bag systems includes:
Process control of our fermenters
Data acquisition, monitoring and control of fermentation processes.
BioPAT® MFCS/win is a Windows-based SCADA software for control, data acquisition and data processing from fermentation processes used all over the world. The GAMP 4 category system can meet basically all specific wishes and requirements. It is modularly built, enabling you to choose the features you need:
Recipe Control:
Allows you to program reproducible fermentation processes.
21CFR Part 11:
Expands the MFCS/win SCADA system to meet FDA’s 21CFR Part 11 guidelines.
Terminal Server: Allows connection to MFCS/win server from office or home PC (workstation). Remote Alarming: Transfers alarms to mobile phone or email.
The most important feature of the system is of course acquisition of fermentation data for later analysis purposes. If you wish to control fermentations via a computer, this is possible with the recipe module (S88-recipe) with which the whole fermentation process can be preprogrammed.
Courses in BioPAT® MFCS/win
We offer BioPAT® MFCS/win courses. When these are to be held are determined individually. To learn more about our training program click on the links below.
Basic Course BioPAT® MFCS/win 3.1 User Training Program
BioPAT® MFCS 4 User Training Program
Sartorius also offers remote training. To learn more about this click on the links below.
Feel free to contact us – we are ready to help you with bioreactors and fermenters
Søren Bidstrup
Managing Director and product specialist
Email: sb@aagechristensen.dk
Mobile: +45 40 45 60 10
